Research Paper
Genetic diversity and conservation of Carex kobomugi in Taiwan
Chi-Chun Huang, Chao-Yu Hsu, Kuo-Hsiang Hung, Tsai-Wen Hsu, Chau-Ching Huang, Munkhtsetseg Tsednee, Chuan-Ming Yeh, Wei-Kuang Wang
Published on: 24 June 2025
Page: 417 - 431
DOI: 10.6165/tai.2025.70.417
Abstract
Carex kobomugi is native to the coastal sand dunes and sandy beaches of East Asia, including China, Japan, Korea, Taiwan, and the Russian Far East. In Taiwan, C. kobomugi, which is categorized as threatened species (category of critically endangered, CR), is narrowly distributed in northern Taiwan and offshore islands. First recorded in 1924, the species disappeared for a time but was rediscovered in 2020. However, it is currently threatened by increasing human activities and faces the risk of local extinction. In this study, we employed molecular markers (rpL16 spacer of cpDNA, ITS region of nrDNA and eleven microsatellite loci) to evaluate the genetic diversity and population structure. The results showed that the low genetic diversity of C. kobomugi in Taiwan may be attributed to small population size, and geographic isolation based on cpDNA and nrDNA. In contrast to the identical nrDNA sequences across different regions, a unique cpDNA haplotype identified exclusively in Taiwan underscores the need for focused conservation efforts. A moderate level of genetic diversity was found for microsatellite loci, and STRUCTURE and principal coordinates analysis revealed that the two genetic groups corresponded to their geographical distributions. Based on these findings, two distinct management units are recommended for further conservation. Actions such as manually conducted beach cleanups, species inventories, and both in-situ and ex-situ conservation have been implemented to mitigate human disturbance.
Keyword: Carex kobomugi, conservation, ITS, microsatellite, population genetics, rpL16
Literature Cited
Bouckaert, R., Vaughan, T.G., Barido-Sottani, J., Duch?ne, S., Fourment, M., Gavryushkina, A., Heled, J., Jones, G., K?hnert, D., DeMaio, N., Matschiner, M., Mendes, F.K., M?ller, N.F., Ogilvie, H.A., DuPlessis, L., Popinga, A., Rambaut, A., Rasmussen, D., Siveroni, I., Suchard, M., Wu, C.H., Xie, D., Zhang, C., Stadler, T., Drummond, A.J. 2019 BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis. PLoS Comput. Biol. 15(4): e1006650.
DOI: 10.1371/journal.pcbi.1006650View Article
Google Scholar
Chang, C.T., Tsai, C.N., Tang, C.Y., Chen, C.H., Lian, J.H., Hu, C.Y., Tsai, C.L., Chao, A., Lai, C.H., Wang, T.H., Lee, Y.S. 2012 Mixed sequence reader: a program for analyzing DNA sequences with heterozygous base calling. Sci. World J. 2012(1): 365104.
DOI: 10.1100/2012/365104View Article
Google Scholar
Channell, R. 2004 The conservation value of peripheral populations?: the supporting science. In: T. D.Hooper (eds.), Proceedings of the species at risk 2004 pathways to recovery conference. 1–17. Species at Risk 2004 Pathways to Recovery Conference Organizing Committee, Victoria, BC.
Charbonneau, B.R., Nicoletta, R., Wootton, L.S. 2020 A decade of expansion of the invasive plant Carex kobomugi in a coastal foredune system. Biol. Invasions 22(6): 2099–2112.
DOI: 10.1007/s10530-020-02240-6View Article
Google Scholar
Chen, S.Y., Huang, C.C., Cheng, Y.T., Wang, C.C., Li, C.Y., Lai, I.L., Hung, K.H. 2023 Effect of geographic isolation on genetic variation and population structure of Euphrasia nankotaizanensis, a threatened endemic alpine herb in Taiwan. Heliyon 9(3): e14228.
DOI: 10.1016/j.heliyon.2023.e14228View Article
Google Scholar
Chiang, T.Y., Chiang, Y.C., Chen, Y.J., Chou, C.H., Havanond, S., Hong, T.N., Huang, S. 2001a Phylogeography of Kandelia candel in East Asiatic mangroves based on nucleotide variation of chloroplast and mitochondrial DNAs. Mol. Ecol. 10(11): 2697–2710.
DOI: 10.1046/j.0962-1083.2001.01399.xView Article
Google Scholar
Chiang, T.Y., Hong, K.H., Peng, C.I. 2001b Experimental hybridization reveals biased inheritance of the internal transcribed spacer of the nuclear ribosomal DNA in Begonia ×taipeiensis. J. Plant Res., 114(3), 343–351.
DOI: 10.1007/PL00013996View Article
Google Scholar
Chiang, T.Y., Schaal, B.A. 2006 Phylogeography of plants in Taiwan and the Ryukyu Archipelago. Taxon 55(1): 31–41.
DOI: 10.2307/25065526View Article
Google Scholar
Clugston, J.A.R., Coolen, Q., Houtepen, E., Van Proosdij, A. S.J., Grinage, A.D., Griffith, M.P. 2024 Genomic patterns of native palms from the Leeward Antilles confirm single-island endemism and guide conservation priorities. Conser. Genet. 25(4): 985–997.
DOI: 10.1007/s10592-024-01618-6View Article
Google Scholar
Coates, D.J., Byrne, M., Moritz, C. 2018 Genetic diversity and conservation units: Dealing with the species-population continuum in the age of genomics. Front. Ecol. Evol. 6: 165.
DOI: 10.3389/fevo.2018.00165View Article
Google Scholar
Dai, X., Wu, W., Ji, L., Tian, S., Yang, B., Guan, B., Wu, D. 2022 MaxEnt model-based prediction of potential distributions of Parnassia wightiana (Celastraceae) in China. Biodivers. Data J. 10: e81073.
DOI: 10.3897/BDJ.10.e81073View Article
Google Scholar
Darriba, D., Taboada, G.L., Doallo, R., Posada, D. 2012 JModelTest 2: More models, new heuristics and parallel computing. Nat. Methods 9(8): 772.
DOI: 10.1038/nmeth.2109View Article
Google Scholar
Eckstein, R.L., O’Neill, R.A., Danihelka, J., Otte, A., K?hler, W. 2006 Genetic structure among and within peripheral and central populations of three endangered floodplain violets. Mol. Ecol. 15(9): 2367–2379.
DOI: 10.1111/j.1365-294X.2006.02944.xView Article
Google Scholar
Editorial Committee of the Red List of Taiwan Plants 2017 The Red List of Vascular Plants of Taiwan, Endemic Species Research Institute, Forestry Burea. 187 pp.
Ellstrand, N.C. 1993 Population genetic consequences of small population size: implications for plant conservation. Annu. Rev. Ecol. Syst. 24(1): 217–242.
DOI: 10.1146/annurev.es.24.110193.001245View Article
Google Scholar
Escudero, M., Weber, J.A., Hipp, A.L. 2013 Species coherence in the face of karyotype diversification in holocentric organisms: the case of a cytogenetically variable sedge (Carex scoparia, Cyperaceae). Ann. Bot. 112(3): 515–526.
DOI: 10.1093/aob/mct119View Article
Google Scholar
Excoffier, L., Lischer, H.E.L. 2010 Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Resour., 10(3): 564–567.
DOI: 10.1111/j.1755-0998.2010.02847.xView Article
Google Scholar
Fan, Y., Dai, J., Wei, Y., Liu, J. 2023.Local adaptation in natural populations of Toona ciliata var. pubescens is driven by precipitation and temperature: evidence from microsatellite markers. Forests 14(10): 1998.
DOI: 10.3390/f14101998View Article
Google Scholar
Feng, X., Wang, Y., Gong, X. 2014 Genetic diversity, genetic structure and demographic history of Cycas simplicipinna (Cycadaceae) assessed by DNA sequences and SSR markers. BMC Plant Biol. 14(1): 187.
DOI: 10.1186/1471-2229-14-187View Article
Google Scholar
Ford, B.A., Iranpour, M., Naczi, R.F.C., Starr, J.R., Jerome, C.A. 2006 Phylogeny of Carex subg. Vignea (Cyperaceae) based on non-coding nrDNA sequence data. Syst. Bot. 31(1): 70–82.
DOI: 10.1600/036364406775971813View Article
Google Scholar
Fournier-Level, A., Korte, A., Cooper, M.D., Nordborg, M., Schmitt, J., Wilczek, A.M. 2011 A map of local adaptation in Arabidopsis thaliana. Science, 334(6052): 86–89.
DOI: 10.1126/science.1209271View Article
Google Scholar
Furlan, E., Stoklosa, J., Griffiths, J., Gust, N., Ellis, R., Huggins, R.M., Weeks, A.R. 2012 Small population size and extremely low levels of genetic diversity in island populations of the platypus, Ornithorhynchus anatinus. Ecol. and Evol. 2(4): 844–857.
DOI: 10.1002/ece3.195View Article
Google Scholar
Garc?a-Ramos, G., Kirkpatrick, M. 1997 Genetic models of adaptation and gene flow in peripheral populations. Evolution 51(1): 21–28.
DOI: 10.2307/2410956View Article
Google Scholar
Garner, K.L., Chang, M.Y., Fulda, M.T., Berlin, J. ., Freed, R.E., Soo-Hoo, M.M., Revell, D.L., Ikegami, M., Flint, L. E., Flint, A.L., Kendall, B.E. 2015 Impacts of sea level rise and climate change on coastal plant species in the central California coast. PeerJ 2015(5): e958.
DOI: 10.7717/peerj.958View Article
Google Scholar
Ge, X.J., Hsu, T.W., Hung, K.H., Lin, C.J., Huang, C.C., Huang, C.C., Chiang, Y.C., Chiang, T.Y. 2012 Inferring multiple refugia and phylogeographical patterns in Pinus massoniana based on nucleotide sequence variation and DNA fingerprinting. PLoS ONE 7(8): e43717.
DOI: 10.1371/journal.pone.0043717View Article
Google Scholar
Geng, Q., Wang, Z., Tao, J., Kimura, M.K., Liu, H., Hogetsu, T., Lian, C. 2021 Ocean currents drove genetic structure of seven dominant mangrove species along the coastlines of southern China. Front. Genet. 12: 615911.
DOI: 10.3389/fgene.2021.615911View Article
Google Scholar
Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O. 2010 New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst. Biol. 59(3): 307–321.
DOI: 10.1093/sysbio/syq010View Article
Google Scholar
Han, C.C., Lai, C.H., Huang, C.C., Wang, I.C., Lin, H.D., Wang, W.K. 2022 Phylogeographic Structuring of the Kuroshio-Type Prawn Macrobrachium japonicum (Decapoda: Palaemonidae) in Taiwan and Ryukyu Islands. Diversity 14(8): 617.
DOI: 10.3390/d14080617View Article
Google Scholar
Hijmans, R., Phillips, S., Leathwick, J., Elith, J. 2024 dismo: species distribution modeling. R Package Version 1.3-16.
Hsieh, C.-F., Shen, C.-F., Yang, K.-C. 1994 Introduction to the flora of Taiwan, 3: floristics, phytogeography, and vegetation. In: E. C. of the F. of Taiwan (Eds), Flora of Taiwan, 2nd ed. 2: 7–18). Department of Botany, National Taiwan University.
Hu, L., Wang, J., Wang, X., Zhang, D., Sun, Y., Lu, T., Shi, W. 2024 Development of SSR markers and evaluation of genetic diversity of endangered Plant Saussurea involucrata. Biomolecules 14(8): 1010.
DOI: 10.3390/biom14081010View Article
Google Scholar
Huang, B.H., Ruan, Y., Li, J.Q., Liao, P.C. 2015 Applying effective population size estimates of Kandelia obovata Sheue, Liu and Yong to conservation and restoration management. Forests 6(5): 1439–1453.
DOI: 10.3390/f6051439View Article
Google Scholar
Huang, C.C., Hsu, T.W. 2023 Low genetic diversity of the vulnerable Ormocarpum cochinchinense (Fabaceae: Papilionoideae) in Taiwan and implications for its conservation (abstract in English, text in Chinese). Taiwan J. Biodivers. 25(2): 37–54.
Huang, C.C., Hung, K.H., Wang, W.K., Ho, C.W., Huang, C.L., Hsu, T.W., Osada, N., Hwang, C.C., Chiang, T.Y. 2012 Evolutionary rates of commonly used nuclear and organelle markers of Arabidopsis relatives (Brassicaceae). Gene 499(1): 194–201.
DOI: 10.1016/j.gene.2012.02.037View Article
Google Scholar
Huelsenbeck, J.P., Ronquist, F. 2001 MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17(8): 754–755.
DOI: 10.1093/bioinformatics/17.8.754View Article
Google Scholar
Ishikawa, S.I., Furukawa, A., Okuda, T., Oikawa, T. 1993 Germination requirements in Carex kobomugi (Sea Isle). J. Plant Res. 106(3): 245–248.
DOI: 10.1007/BF02344592View Article
Google Scholar
Kamvar, Z.N., Tabima, J.F., Gr?unwald, N.J. 2014 Poppr: An R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction. PeerJ 2014(1): e281.
DOI: 10.7717/peerj.281View Article
Google Scholar
Kardos, M., Armstrong, E.E., Fitzpatrick, S.W., Hauser, S., Hedrick, P.W., Miller, J. M., Tallmon, D.A., Chris Funk, W. 2021 The crucial role of genome-wide genetic variation in conservation. Proc. Natl. Acad. Sci. U.S.A. 118(48): e2104642118.
DOI: 10.1073/pnas.2104642118View Article
Google Scholar
Katoh, K., Rozewicki, J., Yamada, K.D. 2019 MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief. Bioinform. 20(4): 1160–1166.
DOI: 10.1093/bib/bbx108View Article
Google Scholar
Kay, K.M., Whittall, J.B., Hodges, S.A. 2006 A survey of nuclear ribosomal internal transcribed spacer substitution rates across angiosperms: An approximate molecular clock with life history effects. BMC Evol. Biol. 6: 36.
DOI: 10.1186/1471-2148-6-36View Article
King, M.G., Horning, M.E., Roalson, E.H. 2009 Range persistence during the last glacial maximum: Carex macrocephala was not restricted to glacial refugia. Mol. Ecol., 18(20): 4256–4269.
DOI: 10.1111/j.1365-294X.2009.04280.xView Article
Google Scholar
King, M.G., Roalson, E.H. 2008 Exploring evolutionary dynamics of nrDNA in Carex subgenus Vignea (cyperaceae). Syst. Bot. 33(3): 514–524.
DOI: 10.1600/036364408785679860View Article
Google Scholar
King, M.G., Roalson, E.H. 2009 Discordance between phylogenetics and coalescent-based divergence modelling: Exploring phylogeographic patterns of speciation in the Carex macrocephala species complex. Mol. Ecol. 18(3): 468–482.
DOI: 10.1111/j.1365-294X.2008.04033.xView Article
Google Scholar
Ko, Y.Z., Shih, H.C., Ho, C.S., Chen, C.T., Hsu, T.W., Shiao, M.S., Chiang, Y.C. 2024 Assessment of genetic conservation units of an endangered glacial relict insular species, Amentotaxus formosana, based on fine-scale genetic structures of multiple fragmented mountainous populations in Taiwan. Front. Plant Sci., 15: 1–16.
DOI: 10.3389/fpls.2024.1512914View Article
Google Scholar
Lammi, A., Siikam?ki, P., Mustaj?rvi, K. 1999 Genetic diversity, population size, and fitness in central and peripheral populations of a rare plant Lychnis viscaria. Conser. Biol. 13(5): 1069–1078.
DOI: 10.1046/j.1523-1739.1999.98278.xView Article
Google Scholar
Leigh, J.W., Bryant, D. 2015 POPART: Full-feature software for haplotype network construction. Methods Ecol. Evol. 6(9): 1110–1116.
DOI: 10.1111/2041-210X.12410View Article
Google Scholar
Lesica, P., Allendorf, F.W. 1995 When are peripheral populations valuable for conservation? Conserv. Biol. 9(4): 753–760.
DOI: 10.1046/j.1523-1739.1995.09040753.xView Article
Google Scholar
Li, Y.L., Liu, J.X. 2018 StructureSelector: A web-based software to select and visualize the optimal number of clusters using multiple methods. Mol. Ecol. Resour. 18(1): 176–177.
DOI: 10.1111/1755-0998.12719View Article
Google Scholar
Liao, W., Hu, J., Peng, P. 2018 Burial of organic carbon in the Taiwan Strait. Journal of Geophysical Research: Oceans, 123(9): 6639–6652.
DOI: 10.1029/2018JC014285View Article
Google Scholar
Liu, C., Wang, J., Ko, Y. Z., Shiao, M. S., Wang, Y., Sun, J., Yuan, Q., Wang, L., Chiang, Y. C., Guo, L. 2024 Genetic diversities in wild and cultivated populations of the two closely-related medical plants species, Tripterygium Wilfordii and T. Hypoglaucum (Celastraceae). BMC Plant Biology 24(1): 195.
DOI: 10.1186/s12870-024-04826-xView Article
Google Scholar
M’Baya, J., Blacket, M.J., Hoffmann, A.A. 2013 Genetic structure of Carex species from the Australian alpine region along elevation gradients: Patterns of reproduction and gene flow. Int. J. Plant Sci. 174(2): 189–199.
DOI: 10.1086/668793View Article
Google Scholar
Madsen, T., Shine, R., Olsson, M., Wittzell, H. 1999 Restoration of an inbred adder population. Nature 402(6757): 34–35.
DOI: 10.1038/46941View Article
Google Scholar
Marshall, T.C., Slate, J., Kruuk, L. E. B., Pemberton, J.M. 1998 Statistical confidence for likelihood-based paternity inference in natural populations. Mol. Ecol. 7(5): 639–655.
DOI: 10.1046/j.1365-294x.1998.00374.xView Article
Google Scholar
Miryeganeh, M., Takayama, K., Tateishi, Y., Kajita, T. 2014 Long-distance dispersal by sea-drifted seeds has maintained the global distribution of Ipomoea pes-caprae subsp. brasiliensis (convolvulaceae). PLoS ONE 9(4), e91836.
DOI: 10.1371/journal.pone.0091836View Article
Google Scholar
Morjan, C.L., Rieseberg, L.H. 2004 How species evolve collectively: Implications of gene flow and selection for the spread of advantageous alleles. Mol. Ecol. 13(6): 1341–1356.
DOI: 10.1111/j.1365-294X.2004.02164.xView Article
Google Scholar
Murray, M.G., Thompson, W.F. 1980 Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res., 8(19): 4321–4325.
DOI: 10.1093/nar/8.19.4321View Article
Google Scholar
Nei, M. 1987 Molecular Evolutionary Genetics. Columbia University Press, New York.
DOI: 10.7312/nei-92038View Article
Nikolic, N., Chevalet, C. 2014 Detecting past changes of effective population size. Evol. Appl., 7(6): 663–681.
DOI: 10.1111/eva.12170View Article
Google Scholar
Ochoa-Zavala, M., Osorio-Olvera, L., Pi?ero, D., N??ez-Farf?n, J. 2020 Inferring potential barriers to gene flow in tropical populations of Avicennia germinans. Aquat. Bot. 161: 103170.
DOI: 10.1016/j.aquabot.2019.103170View Article
Google Scholar
Ohsako, T. 2010 Clonal and spatial genetic structure within populations of a coastal plant, Carex Kobomugi (Cyperaceae). Am. J. Bot. 97(3): 458–470.
DOI: 10.3732/ajb.0900262View Article
Google Scholar
Ohsako, T., Yamane, K. 2007 Isolation and characterization of polymorphic microsatellite loci in Asiatic sand sedge, Carex kobomugi Ohwi (Cyperaceae). Mol. Ecol. Notes 7(6): 1023–1025.
DOI: 10.1111/j.1471-8286.2007.01761.xView Article
Google Scholar
Palsb?ll, P.J., B?rub?, M., Allendorf, F.W. 2007 Identification of management units using population genetic data. Trends Ecol. Evol. 22(1): 11–16.
DOI: 10.1016/j.tree.2006.09.003View Article
Google Scholar
Peakall, R., Smouse, P.E. 2012 GenALEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28(19): 2537–2539.
DOI: 10.1093/bioinformatics/bts460View Article
Google Scholar
Phillips, S.J., Anderson, R.P., Schapire, R.E. 2006 Maximum entropy modeling of species geographic distributions. Ecol. Modell. 190(3–4): 231–259.
DOI: 10.1016/j.ecolmodel.2005.03.026View Article
Google Scholar
Pritchard, J.K., Stephens, M., Donnelly, P. 2000 Inference of population structure using multilocus genotype data. Genetics 155(2): 945–959.
DOI: 10.1093/genetics/155.2.945View Article
Google Scholar
Rambaut, A. 2018 Molecular evolution, phylogenetics and epidemiology. FigTree ver. 1.4.4 software. Available online at: http://tree.bio.ed.ac.uk/software/figtree/.
Rambaut, A., Drummond, A. J., Xie, D., Baele, G., Suchard, M. A. 2018 Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Syst. Biol. 67(5): 901–904.
DOI: 10.1093/sysbio/syy032View Article
Google Scholar
Rana, S.K., Rana, H.K., Ghimire, S.K., Shrestha, K.K., Ranjitkar, S. 2017 Predicting the impact of climate change on the distribution of two threatened Himalayan medicinal plants of Liliaceae in Nepal. J. Mt. Sci. 14(3): 558–570.
DOI: 10.1007/s11629-015-3822-1View Article
Google Scholar
Riffe, E.C., Zinnert, J.C. 2024 Impact of invasive Carex kobomugi on the native dune community in a US mid-Atlantic coastal system. Biol. Invasions, 26(4): 1195–1208.
DOI: 10.1007/s10530-023-03237-7View Article
Google Scholar
Roalson, E.H., Friar, E.A. 2004 Phylogenetic relationships and biogeographic patterns in North American members of Carex section Acrocystis (Cyperaceae) using nrDNA ITS and ETS sequence data. Plant Syst. Evol., 243(3–4): 175–187.
DOI: 10.1007/s00606-003-0089-8View Article
Google Scholar
Rozas, J., Ferrer-Mata, A., Sanchez-DelBarrio, J.C., Guirao-Rico, S., Librado, P., Ramos-Onsins, S.E., Sanchez-Gracia, A. 2017 DnaSP 6: DNA sequence polymorphism analysis of large data sets. Mol. Biol. Evol. 34(12): 3299–3302.
DOI: 10.1093/molbev/msx248View Article
Google Scholar
Ruan, Y., Huang, B.H., Lai, S.J., Wan, Y.T., Li, J.Q., Huang, S., Liao, P.C. 2013 Population genetic structure, local adaptation, and conservation genetics of Kandelia obovata. Tree Genet. Genomes 9(4): 913–925.
DOI: 10.1007/s11295-013-0605-0View Article
Google Scholar
Savolainen, O., Lascoux, M., Meril?, J. 2013 Ecological genomics of local adaptation. Nat. Rev. Genet. 14(11): 807–820.
DOI: 10.1038/nrg3522View Article
Google Scholar
Savolainen, O., Pyh?j?rvi, T., Kn?rr, T. 2007 Gene flow and local adaptation in trees. Annu.l Rev. Ecol. Evol. Syst. 38(1): 595–619.
DOI: 10.1146/annurev.ecolsys.38.091206.095646View Article
Google Scholar
Schlotterer, C. 2000 Evolutionary dynamics of microsatellite DNA. Chromosoma 109(6): 365–371.
DOI: 10.1007/s004120000089View Article
Google Scholar
Sch?nswetter, P., Elven, R., Brochmann, C. 2008 Trans?Atlantic dispersal and large?scale lack of genetic structure in the circumpolar, arctic-alpine sedge Carex bigelowii s. l. (Cyperaceae). Am. J. Bot. 95(8): 1006–1014.
DOI: 10.3732/ajb.2007196View Article
Google Scholar
Senni, K., Fuji, N., Takhashi, H. 2005 Intraspecific chloroplast DNA variations of the alpine plants in Japan. Acta Phytotaxon. Geobot. 56(3): 265–275.
Sexton, J.P., Strauss, S.Y., Rice, K.J. 2011 Gene flow increases fitness at the warm edge of a species’ range. Proc. Natl. Acad. Sci. U.S.A. 108(28): 11704–11709.
DOI: 10.1073/pnas.1100404108View Article
Google Scholar
Shaw, J., Lickey, E. B., Beck, J. T., Farmer, S. B., Liu, W., Miller, J., Siripun, K. C., Winder, C. T., Schilling, E. E., Small, R. L. 2005 The tortoise and the hare II: Relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. Am. J. Bot. 92(1): 142–166.
DOI: 10.3732/ajb.92.1.142View Article
Google Scholar
Su?rez, D., Arribas, P., Jim?nez-Garc?a, E., Emerson, B.C. 2022 Dispersal ability and its consequences for population genetic differentiation and diversification. Proc. R. Soc. B Biol. Sci. 289(1975): 289.
DOI: 10.1098/rspb.2022.0489View Article
Google Scholar
Takayama, K., Kajita, T., Murata, J., Tateishi, Y. 2006 Phylogeography and genetic structure of Hibiscus tiliaceus-Speciation of a pantropical plant with sea-drifted seeds. Mol. Ecol. 15(10): 2871–2881.
DOI: 10.1111/j.1365-294X.2006.02963.xView Article
Google Scholar
Takayama, K., Tateishi, Y., Murata, J., Kajita, T. 2008 Gene flow and population subdivision in a pantropical plant with sea-drifted seeds Hibiscus tiliaceus and its allied species: Evidence from microsatellite analyses. Mol. Ecol. 17(11): 2730–2742
DOI: 10.1111/j.1365-294X.2008.03799.xView Article
Google Scholar
Wahlsteen, E. 2021 SSR markers distinguish critically endangered Acer campestre populations from cryptic invading gene pools. Willdenowia 51(1): 115–125.
DOI: 10.3372/wi.51.51109View Article
Google Scholar
Wang, J.C., Chen, H.H., Hsu, T.W., Hung, K.H., Huang, C.C. 2024 A taxonomic revision of the genus Angelica (Apiaceae) in Taiwan with a new species A. aliensis. Bot. Stud. 65(1): 3.
DOI: 10.1186/s40529-023-00407-7View Article
Google Scholar
Wee, A.K.S., Takayama, K., Asakawa, T., Thompson, B., Onrizal, Sungkaew, S., Tung, N.X., Nazre, M., Soe, K.K., Tan, H.T. W., Watano, Y., Baba, S., Kajita, T., Webb, E. L. 2014 Oceanic currents, not land masses, maintain the genetic structure of the mangrove Rhizophora mucronata Lam. (Rhizophoraceae) in Southeast Asia. J. Biogeogr. 41(5): 954–964.
DOI: 10.1111/jbi.12263View Article
Google Scholar
WFO 2024. Carex kobomugi Ohwi. Published on the Internet;Http://Www.Worldfloraonline.Org/Taxon/Wfo-0000347956. Accessed on: 02 Aug 2024.
Willyard, A., Syring, J., Gernandt, D. S., Liston, A., Cronn, R. 2007. Fossil calibration of molecular divergence infers a moderate mutation rate and recent radiations for Pinus. Mol. Biol. Evol. 24(1): 90–101.
DOI: 10.1093/molbev/msl131View Article
Google Scholar
Wilson, G.A., Rannala, B. 2003 Bayesian inference of recent migration rates using multilocus genotypes. Genetics, 163(3): 1177–1191.
DOI: 10.1093/genetics/163.3.1177View Article
Google Scholar
Wolfe, K.H., Li, W.H., Sharp, P.M. 1987 Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proc. Natl. Acad. Sci. U.S.A. 84(24): 9054–9058.
DOI: 10.1073/pnas.84.24.9054View Article
Google Scholar
Wootton, L.S., Halsey, S.D., Bevaart, K., McGough, A., Ondreicka, J., Patel, P. 2005 When invasive species have benefits as well as costs: Managing Carex kobomugi (Asiatic sand sedge) in New Jersey’s coastal dunes. Biol. Invasions 7(6): 1017–1027.
DOI: 10.1007/s10530-004-3124-yView Article
Google Scholar
Yamamoto, T., Tsuda, Y., Takayama, K., Nagashima, R., Tateishi, Y., Kajita, T. 2019 The presence of a cryptic barrier in the West Pacific Ocean suggests the effect of glacial climate changes on a widespread sea-dispersed plant, Vigna marina (Fabaceae). Ecol. Evol. 9(15): 8429–8440.
DOI: 10.1002/ece3.5099View Article
Google Scholar
Yan, H., Qi, H., Li, Y., Wu, Y., Wang, Y., Chen, J., Yu, J. 2022 Assessment of the genetic relationship and population structure in oil-Tea Camellia species using simple sequence repeat (SSR) markers. Genes, 13(11): 2162.
DOI: 10.3390/genes13112162View Article
Google Scholar
Yang, H., Lu, Q., Wu, B., Zhang, J. 2012 Seed dispersal of East Asian coastal dune plants via seawater - short and long distance dispersal. Flora Morphol. Distrib. Funct. Ecol. Plants 207(10): 701–706.
DOI: 10.1016/j.flora.2012.08.001View Article
Google Scholar
Yano, O., Ikeda, H., Hoshino, T. 2010 Phylogeography of the Japanese common sedge, Carex conica complex (Cyperaceae), based on chloroplast DNA sequence data and chromosomal variation. Am. J. Bot., 97(8): 1365–1376.
DOI: 10.3732/ajb.0900338View Article
Google Scholar
Yu, G. 2020 Using ggtree to visualize sata on tree-like structures. Current Protocols in Bioinforma. 69(1): e96. h
DOI: 10.1002/cpbi.96View Article
Google Scholar
Zhang, X., Liu, Y. H., Wang, Y.H., Shen, S.K. 2020 Genetic diversity and population structure of Rhododendron rex subsp. rex inferred from microsatellite markers and chloroplast DNA sequences. Plants 9(3): 338.
DOI: 10.3390/plants9030338View Article
Google Scholar
Zhang, X., Yang, L., Liu, Y.H., Zhou, X.L., Zhang, L.Q., Wang, Y.H., Shen, S.K. 2021 Genetic diversity, genetic structure, and demographic history of Cinnamomum chago, a plant species with extremely small populations in China. Glob. Ecol. Conserv. 31, e01808.
DOI: 10.1016/j.gecco.2021.e01808View Article
Google Scholar
Zimmerman, S.J., Aldridge, C.L., Oyler-Mccance, S.J. 2020 An empirical comparison of population genetic analyses using microsatellite and SNP data for a species of conservation concern. BMC Genomics 21(1): 1–16.
DOI: 10.1186/s12864-020-06783-9View Article
Google Scholar