Abstract

Nodal explants from aseptic seedlings of Hildergardia populifolia cultured on Murashige and Skoog’s (MS) medium supplemented with 2 mgL-1 N6-benzylaminopurine (BAP) generated very few shoots over a period of 30 days. Shoots were rooted in vitro on 1/2 strength MS macro salts containing 2 mgL-1 naphthalene acetic acid (NAA). A significant achievement in the present investigation was the production of somatic embryos from immature zygotic embryo explants exposed to MS medium supplemented with NAA 3 mgL-1 and casein hydrolysate (CH) 1000 mgL-1 over a period of 60 days. Subsequent withdrawl of NAA and CH from the medium resulted in the maturation and growth of embryos into plantlets on MS medium. The plantlets were transferred to sand: manure: soil mixture (1:1:1) where they showed no visual abnormalities in morphological characters.

中文摘要

從Hildegardia populiforia幼苗取其節的片段組織培養於含有2 mgL-1 BAP的Marashige and Skoog’s (MS)培養劑中，經30天後即有少數枝條的產生。這些枝條再培養於含有2 mgL-1 NAA並且已稀釋一倍的MS培養劑中，則可長出根來。若把不成熟的接合子胚的片段組織培養於含有3 mgL-1 NAA及1000 mgL-1乳蛋白水解物(CH)的MS培養劑中，經60天後即可產生體胚。若是把NAA及CH從培養劑中去除，此體胚則可長成小樹，此小樹再移植於沙/糞肥/土(1:1:1)的混合土壤中生長，並沒有發現其外部型態的特徵有不正常之現象。

Keyword: Hildegardia populifolia, Endangered tree, Somatic embryogenesis, Nodal regeneration.