Abstract

Tobacco protoplast enzymatically isolated from suspension culture were studied. Isolated protoplast showed intact plasma membrane surrounded the outer surface, but sometimes membrane with breb was also observed. Endoplasmic reticulum was abundant and 3 to 10 layers of cisternae in parallel arrangement ofren occurred. Invaginations of cytoplasmic membrane with sime organelles in it were also found. Oil drops prominently occurred in isolated protoplasts with different characters in staining properties and structure. The central and large vacuoles disappeared and small vacuoles tremendously increased in number. Nucleus located in the center of protoplasts with a distinct nucleolus. Annuli on the surface of nuclear envelope were numerous (35/um^2). Plastids were different in shape and size. Structural patterns of plastids are more diverse than that of mitochondria. Stereopairs showed the three dimensional structures of protoplasts were all identical to that observed in thin section.

中文摘要

振盪培養細胞經以酵素法處理可得圓形而無壁的原生質體，經切片或冰蝕法處理可得相互印證，內質網發達板囊常有3~10層，又高基爾體常在其附近出現，不同性質的油滴分別以單一或聚集存在，粒線體形狀穩定而色素體變化較大，大部分的原生質體內都有許多小液泡充塞著而大液泡消失，大而圓的細胞核位於原生質體的中央，原生質體經誘導處理可產生一含兩個以上的核的原生質體，原生質體是研究細胞壁再生的好材料。