Abstract

Active cells were harvested from tobacco cell suspension culture. The acid phosphatase of tobacco cells was mostly located in the cell wall and vacuole. Similar zymogram of acid phosphatase revealed in the gradient polyacrylamide gel was observed between the tobacco leaves and their induced-callus. Multiple acid phosphatases of tobacco suspension cells esperated by chromatography showed similar substrate specificities to most compounds tested. Zn2+ has differential inhibition on the multiple forms of acid phosphatase of tobacco cells.

中文摘要

自煙草懸浮培養中收集之細胞，發現其酸性磷解?大部分位於液泡及細胞壁。煙草葉片及其瘉合組織經由聚丙烯 胺梯度電泳分析，發現其酸性磷解?之同功酵素圖譜類似。而且煙草懸浮培養之細胞中之酸性磷解?以管柱色層分析法可分出多種酸性磷解?。他們具有相似之基質特異性，然而鋅離子對它們卻表現不同程度之抑制。