Research Paper

Thaxterogaster shoreae, a new species of Thaxterogaster subg. Scauri sect. Purpurascentes from Sal Forest of India based on morphology and molecular phylogeny

Aniket Ghosh, Dyutiparna Chakraborty, Manoj E. Hembrom, Alfredo Vizzini, Kanad Das

Published on: 03 January 2023

Page: 23 - 30

DOI: 10.6165/tai.2023.68.23


Routine macrofungal explorations of different Shorea robusta dominated forest areas of Jharkhand and West Bengal are being undertaken since 2020. A specimen classified as "Thaxterogaster" was collected recently from these states and here proposed as Thaxterogaster shoreae sp. nov. within subg. Scauri sect. Purpurascentes. Detailed morphological description, illustrations 2-locus phylogenetic estimations and comparisons with respective allied species are provided. Moreover, in this communication one new combination in the genus Thaxterogaster is also proposed.

Keyword: Basidiomycota, Cortinariaceae, macrofungi, , Shorea robusta, Taxonomy, Thaxterogaster indopurpurascens

Literature Cited

Berkeley, M.J. 1852. Decades of fungi. Decades XXXII, XXXIII. Sikkim Himalaya fungi, collected by Dr. J.D. Hooker. Hooker's J. Bot. Kew Gard. Misc. 3: 39–49.

Bhavanidevi, S., Nair, M.C. 1983. Addition to Indian Agaricales. In: Kaul, T. N. and B. M. Kapoor (eds.), Indian Mushroom Science-II, CSIR, New Delhi. pp. 271– 274.

Buyck, B., Duhem, B., Das, K., Jayawardena, R.S., Niveiro, N., Pereira, O.L., Prasher, I.B., Adhikari, S., Albert?, E.O., Bulgakov, T.S., Casta?eda-Ruiz, R.F., Hembrom, M.E., Hyde, K.D., Lewis, D.P., Michlig, A., Nuytinck, J., Parihar, A., Popoff, O.F., Ramirez, N.A., da Silva, M., Verma, R.K, Valerie, H. 2017. Fungal Biodiversity Profiles 21?30. Cryptogam. Mycol. 38(1): 101–146.
DOI: 10.7872/crym/v38.iss1.2017.101View Article Google Scholar

Chona, B.L., Lal, G., Kakria, N.C. 1958. The fungi of Delhi. ICAR Bulletin No. 81. 43 pp.

Crous, P.W., Cowan, D.A., Maggs-K?lling, G., Yilmaz, N., Larsson, E., Angelini, C., Brandrud, T.E., et al. 2020. Fungal Planet description sheets: 1112–1181. Persoonia 45(1): 402–403.
DOI: 10.3767/persoonia.2020.45.10View Article Google Scholar

Das, K., Chakraborty, D. 2014. Cortinarius flammeus Berk. - a poorly known species. NeBIO 5: 9–12.

Das, K., Chakraborty, D. 2015. Two new records of Cortinarius from Sikkim (India). J. New Biol. reports 4: 1–6.
DOI: 10.36036/MR.30.1.2021.116219View Article Google Scholar

Das, K., Chakraborty, D., Ghosh, A., Vizzini, A. 2022. Cortinarius dombangensis sp. nov. and C. longistipitatus (Agaricales, Cortinariaceae) from Indian Himalaya. Nord. J. Bot. 2022(6): e03579: 1–11.
DOI: 10.1111/njb.03579View Article Google Scholar

Das, K., Chakraborty, D., Parihar, A. 2013. Cortinarius trivialis, a new record of wild mushroom showing intercontinental conspecificity. NeBIO 4(5): 91–93.

Drummond, A. J., Ashton, B., Buxton, S., Cheung, M., Cooper, A., Heled, J., Kearse, M., Moir, R., Stones-Havas, S., Sturrock, S., Thierer, T., Wilson, A. 2010. Geneious v. 5.1. Available from

Edler, D., Klein, J., Antonelli, A., Silvestro, D. 2021. raxmlGUI 2.0: a graphical interface and toolkit for phylogenetic analyses using RAxML. Methods Ecol. Evol. 12(2): 373–377.
DOI: 10.1111/2041-210X.13512View Article Google Scholar

Florence, E.J.M. 2004. Biodiversity Documentation for Kerala, Part 2: Microorganisms (Fungi). KFRI Handbook No.17. Kerala Forest Research Institute, Peechi, Kerala, India.

Gardes, M., Bruns, T.D. 1993. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol. Ecol. 2(2): 113–118.
DOI: 10.1111/j.1365-294X.1993.tb00005.xView Article Google Scholar

Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.

Hembrom, M. E., K. Das, S. Adhikari, A. Parihar and B. Buyck 2017. First report of Pterygellus from Rajmahal hills of Jharkhand (India) and its relation to Craterellus (Hydnaceae, Cantharellales). Phytotaxa 306(3): 201–210.
DOI: 10.11646/phytotaxa.306.3.2View Article Google Scholar

Itoo, Z. A., Reshi, Z. A., Basharat, Q., Majeed, S.T., Andrabi, K.I. 2015. Identification and characterization of Ectomycorrhizal Cortinarius species (Agaricales, Basidiomycetes) from temperate Kashmir Himalaya, India, by ITS barcoding. Advances in Molecular Biology 2015: 1–9.
DOI: 10.1155/2015/507684View Article Google Scholar

Kalichman, J., Kirk, P.M., Matheny, P.B. 2020. A compendium of generic names of agarics and Agaricales. Taxon 69(3): 425–447.
DOI: 10.1002/tax.12240View Article Google Scholar

Katoh, K., Rozewicki, R., Yamada, K.D. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief. Bioinformatics 20(4): 1160–1166.
DOI: 10.1093/bib/bbx108View Article Google Scholar

Kibby, G. 2015. No. 61 Cortinarius collocandoides. Field Mycol. 16(1): 3–4.
DOI: 10.1016/j.fldmyc.2015.01.003View Article Google Scholar

Kibby, G., Tortelli, M. 2022. The genus Cortinarius in Britain. Ed. G. Kibby and M. Tortelli, UK.

Kornerup, A., Wanscher, J.H. 1978. Methuen handbook of colour, 3rd ed., Eyre Methuen Ltd., London, UK.

Kumar, J., Atri, N.S. 2016. Characterisation of ectomycorrhiza of Russula and Lactifluus (Russulaceae) associated with Shorea robusta from Indian Shiwalik. Nova Hedwigia 103(3–4): 501–513.
DOI: 10.1127/nova_hedwigia/2016/0368View Article Google Scholar

Kumar, J., Atri, N.S. 2019. Characterisation and identification of ectomycorrhizae of Russula (Russulaceae: Basidiomycota) associated with Shorea robusta. J. Trop. For. Sci. 31(1): 114–124.
DOI: 10.26525/jtfs2019.31.1.114124View Article Google Scholar

Kumar, S., Stecher, G., Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7): 1870 –1874.
DOI: 10.1093/molbev/msw054View Article Google Scholar

Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T., Calcott, B. 2017. PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol. Biol. Evol. 34(3): 772 –773.
DOI: 10.1093/molbev/msw260View Article Google Scholar

Liimatainen, K., Kim, J.T., Pokorny, L., Kirk, P.M., Dentinger, B., Niskanen, T. 2022. Taming the beast: a revised classification of Cortinariaceae based on genomic data. Fungal Divers. 112(1): 89–170.
DOI: 10.1007/s13225-022-00499-9View Article Google Scholar

Liimatainen, K., Niskanen, T., Dima, B., Kyt?vuori, I., Ammirati, J.F., Fr?slev, T. G. 2014. The largest type study of Agaricales species to date: bringing identification and nomenclature of Phlegmacium (Cortinarius) into the DNA era. Persoonia 33(1): 98–140.
DOI: 10.3767/003158514X684681View Article Google Scholar

Liimatainen, K., Niskanen, T., Dima, B., Ammirati, J.F., Kirk, P.M., Kyt?vuori, I. 2020. Mission impossible completed: unlocking the nomenclature of the largest and most complicated subgenus of Cortinarius, Telamonia. Fungal Divers. 104(1): 291– 331.
DOI: 10.1007/s13225-020-00459-1View Article Google Scholar

Liu, Y.L., Whelen, S., Hall, B.D. 1999. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Mol. Biol. Evol. 16(12): 1799–1808.
DOI: 10.1093/oxfordjournals.molbev.a026092View Article Google Scholar

Mohanan, C. 2011. Macrofungi of Kerala. Kerala Forest Research Institute, HandBook # 27, Kerala, India, pp. 597.

Natarajan, K., Kumaresan, V., Narayanan, K. 2005. A checklist of Indian agarics and boletes (1984–2002). Kavaka 33: 92.

Parihar, A., Hembrom, M.E., Vizzini, A., Das, K. 2018a. Indoporus shoreae gen. et sp. nov. (Boletaceae) from tropical India. Cryptogam. Mycol. 39(4): 447–466.
DOI: 10.7872/crym/v39.iss4.2018.447View Article Google Scholar

Parihar, A., Hembrom, M.E., Vizzini, A., Das, K. 2018b. A new species of Boletellus (Boletaceae, Basidiomycota) from tropical India. Nord. J. Bot. 36(12): e02089.
DOI: 10.1111/njb.02089View Article Google Scholar

Peintner, U., Moser, M.M., Thomas, K.A., Manimohan, P. 2003. First records of ectomycorrhizal Cortinarius species (Agaricales, Basidiomycetes) from tropical India and their phylogenetic position based on rDNA ITS sequences. Mycol. Res. 107(4): 485–494.
DOI: 10.1017/S0953756203007585View Article Google Scholar

Rambaut, A., Suchard, M.A., Xie, D., Drummond, A.J. 2014. Tracer version 1.6. Available from:

Ronquist, F., Teslenko, M., van der Mark, Ayres, P., D.L., Darling, A., H?hna, S., Larget, B., Liu, L., Suchard, M.A., Huelsenbeck, J.P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61(3): 539–542.
DOI: 10.1093/sysbio/sys029View Article Google Scholar

Saar, G., Dima, B., Schmidt-Stohn, G., Brandrud, T.E., Bell?, F., Fr?slev, T.G., Oertel, B., Soop, K. 2014. Cortinarius Untergattung Phlegmacium Sektion Purpurascentes in Europa. Journal des J.E.C. 14: 140–161.

Sathe, A.V., Daniel, J. 1980. Agaricales (Mushrooms) of Kerala State. MACS Monograph. No. 1. pp. 75–108.

Sathe, A.V., Deshpande, S., Kulkarni, S.M., Daniel, J. 1980. Agaricales (Mushrooms) of South-west India - Part I: Agaricales (Mushrooms) of Maharashtra State (MACS, Pune). pp. 1–114.

Sharma, A.D., Munjal, R.L., Seth, P.K. 1978. Some fleshy fungi from Himachal Pradesh - III. Indian J. Mushr. 4(2): 27–29.

Soop, K., Dima, B., Cooper, J.A., Park, D., Oertel, B. 2019. A phylogenetic approach to a global supraspecific taxonomy of Cortinarius (Agaricales) with an emphasis on the southern Mycota. Persoonia 42(1): 261–290.
DOI: 10.3767/persoonia.2019.42.10View Article Google Scholar

Talavera, G., Castresana, J. 2007. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst. Biol. 56(4): 564–577.
DOI: 10.1080/10635150701472164View Article Google Scholar

Thiers, B. continuously updated. Index Herbariorum: A global directory of public herbaria and associated staff. New York Botanical Garden’s Virtual Herbarium. [accessed 12 September, 2022]

Verma, R. K., Vimal, P., Diwyansh, R., Rao, G.R. 2019. Cortinarius iodes, a New Record from India. Int. J. Curr. Microbiol. Appl. Sci. 8(5): 2306–2311.
DOI: 10.20546/ijcmas.2019.805.272View Article Google Scholar

White, T.J., Bruns, T., Lee, S., Taylor, J.W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M. A., D. H. Gelfand, J. J. Sninsky and T. J. White. (eds.), PCR Protocols: a guide to method and applications. Academic Press, San Diego. pp. 315–322.
DOI: 10.1016/B978-0-12-372180-8.50042-1View Article Google Scholar