Gyalectidium setiferum (Gomphillaceae, Ascomycota), a foliicolous lichen, new to East Asia and its molecular phylogenetic position

Kento Miyazawa, Yoshihito Ohmura, Izumi Okane

Published on: 25 February 2023

Page: 101 - 105

DOI: 10.6165/tai.2023.68.101


Gyalectidium setiferum Vězda & Sérus., a foliicolous lichen characterized by having some vertically oriented whitish translucent cilia surrounding a swollen greenish diahyphal mass, is reported as new to East Asia. It was collected from two localities on the lowlands of central Honshu in Japan where it grew on leaves of Aucuba japonica and Maesa japonica. The description with illustration based on the Japanese material is given. Gyalectidium setiferum has been included in this genus due to its morphological features even though no fertile specimen has been found so far. The monophyly of this species and its position in this genus were newly confirmed by the molecular phylogenetic analysis based on mtSSU and nrLSU.

Keyword: Conidia, conidiomata, diahyphae, distribution, hyphophore, lichenized fungi, mtSSU, nrLSU, photobiont

Literature Cited

Aptroot, A., Ferraro, L.I., Lai, M.J., Sipman, H.J.M., Sparrius, L.B. 2003 Foliicolous lichens and their lichenicolous ascomycetes from Yunnan and Taiwan. Mycotaxon 88: 41–47.

Aptroot, A., Sparrius, L.B. 2003 New microlichens from Taiwan. Fungal Divers 14: 1–50.

Asahina, Y. 1936 Mikrochemischer Nachweis der Flechtenstoffe II. J. Jpn. Bot. 12: 529–536.

Culberson, C.F., Johnson, A. 1982 Substitution of methyl tert-butyl ether for diethyl ether in the standardized thin-layer chromatographic method for lichen products. J. Chromatogr. 238(2): 483–487.
DOI: 10.1016/S0021-9673(00)81336-9View Article Google Scholar

Darienko, T., Gustavs, L., Mudimu, O., Rad Menendez, C., Schumann, R., Karsten, U., Friedl, T., Pr?schold, T. 2010 Chloroidium, a common terrestrial coccoid green alga previously assigned to Chlorella (Trebouxiophyceae, Chlorophyta). Eur. J. Phycol. 45(1): 79–95.
DOI: 10.1080/09670260903362820View Article Google Scholar

Darienko, T., Gustavs, L., Pr?schold, T. 2016 Species concept and nomenclatural changes within the genera Elliptochloris and Pseudochlorella (Trebouxiophyceae) based on an integrative approach. J. Phycol. 52(6): 1125–1145.
DOI: 10.1111/jpy.12481View Article Google Scholar

Darienko, T., Pr?schold, T. 2019 The genus Jaagichlorella Reisigl (Trebouxiophyceae, Chlorophyta) and its close relatives: an evolutionary puzzle. Phytoxaxa 388(1): 47–68.
DOI: 10.11646/phytotaxa.388.1.2View Article Google Scholar

Ferraro, L.I., L?cking, R., S?rusiaux, E. 2001 A world monograph of the lichen genus Gyalectidium (Gomphillaceae). Bot. J. Linn. Soc. 137(3): 311–345.
DOI: 10.1111/j.1095-8339.2001.tb01126.xView Article Google Scholar

Frisch, A., Thor, G., Ertz, D., Grube, M. 2014 The Arthonialean challenge: restructuring Arthoniaceae. Taxon 63(4): 727–744.
DOI: 10.12705/634.20View Article Google Scholar

Herrera-Campos, M.A., L?cking, R. 2003 The foliicolous lichen flora of Mexico II. New species from the montane forest in Oaxaca and Puebla. Bryologist 106(1): 1–8.
DOI: 10.1639/0007-2745(2003)106[0001:TFLFOM]2.0.CO;2View Article Google Scholar

Huss, V.A.R., Frank, C., Hartmann, E.C., Hirmer, M., Kloboucek, A., Seidel, B.M., Wenzeler, P., Kessler, E. 1999 Biochemical taxonomy and molecular phylogeny of the genus Chlorella sensu lato (Chlorophyta). J. Phycol. 35(3): 587–598.
DOI: 10.1046/j.1529-8817.1999.3530587.xView Article Google Scholar

Izumitsu, K., Hatoh, K., Sumita, T., Kitade, Y., Morita, A., Gafur, A., Ohta, A., Kawai, M., Yamanaka, T., Neda, H., Ohta, Y., Tanaka, C. 2012 Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR. Mycoscience 53(5): 396–401.
DOI: 10.1007/S10267-012-0182-3View Article Google Scholar

Katoh, K., Rozwicki, J., Yamada, K.D. 2019 MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Brief. Bioinform. 20(4): 1160–1166.
DOI: 10.1093/bib/bbx108View Article Google Scholar

Krienitz, L., Hegewald, E.H., Hepperle, D., Huss, V.A.R., Rohr, T., Wolf, M. 2004.Phylogenetic relationship of Chlorella and Parachlorella gen. nov. (Chlorophyta, Trebouxiophyceae). Phycologia 43(5): 529–542.
DOI: 10.2216/i0031-8884-43-5-529.1View Article Google Scholar

Krienitz, L., Huss, V.A.R., Bock, C. 2015 Chlorella: 125 years of the green survivalist. Trends Plant Sci. 20(2): 67–69.
DOI: 10.1016/j.tplants.2014.11.005View Article Google Scholar

Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. 2018 MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol. Biol. Evol. 35(6):1547–1549.
DOI: 10.1093/molbev/msy096View Article Google Scholar

L?cking, R. 2008 Foliicolous lichenized Fungi. Flora Neotropica Monograph 103. Organization for Flora Neotropica and The New York Botanical Garden Press, Bronx, New York. 866 pp.

L?cking, R., Buck, W.R., Rivas Plata, E. 2007 The lichen family Gomphillaceae (Ostropales) in eastern North America, with notes on hyphophore development in Gomphillus and Gyalideopsis. Bryologist 110(4): 622–672.
DOI: 10.1639/0007-2745(2007)110[622:TLFGOI]2.0.CO;2View Article Google Scholar

L?cking, R., Stuart, B.L., Lumbsch, H.T. 2004 Phylogenetic relationships of Gomphillaceae and Asterothyriaceae: evidence from a combined Bayesian analysis of nuclear and mitochondrial sequences. Mycologia 96(2): 283–294.
DOI: 10.1080/15572536.2005.11832978View Article Google Scholar

Miadlikowska, J., Lutzoni, F. 2000 Phylogenetic revision of the genus Peltigera (lichen-forming Ascomycota) based on morphological, chemical, and large subunit nuclear ribosomal DNA data. Int. J. Plant Sci. 161(6): 925–958.
DOI: 10.1086/317568View Article Google Scholar

Miyazawa, K., Ohmura, Y., Yamaoka, Y. 2022 Noteworthy foliicolous lichens collected from Iriomote Island, southern Japan. Taiwania 67(1): 155–163.
DOI: 10.6165/tai.2022.67.155View Article Google Scholar

Orange, A., James, P.W., White, F.J. 2001 Microchemical Methods for the Identification of Lichens. British Lichen Society. 101 pp.

Pr?schold, T., Darienko, T., Silva, P.C., Reisser, W., Krienitz, L. 2011 The systematics of “Zoochlorella” revisited employing an integrative approach. Environ. Microbiol. 13(2): 350–364.
DOI: 10.1111/j.1462-2920.2010.02333.xView Article Google Scholar

Safranek, W.W., L?cking, R. 2005 Gyalectidium floridense, a new foliicolous lichen from the southeastern United States. Bryologist 108(2): 295–297.
DOI: 10.1639/0007-2745(2005)108[0295:GFANFL]2.0.CO;2View Article Google Scholar

Sanders, W.B., P?rez-Ortega, S., Nelsen, M.P., L?cking, R., de los R?os, A. 2016 Heveochlorella (Trebouxiophyceae): A little-known genus of unicellular green algae outside the Trebouxiales emerges unexpectedly as a major clade of lichen photobionts in foliicolous communities. J. Phycol. 52(5): 840–853.
DOI: 10.1111/jpy.12446View Article Google Scholar

Schumm, F., Elix, J.A. 2015 Atlas of Images of Thin Layer Chromatograms of Lichen Substances. Books on Demand GmbH, Norderstedt. 584 pp.

S?rusiaux, E. 1993 New taxa of foliicolous lichens from Western Europe and Macaronesia. Nord. J. Bot. 13(4): 447–461.
DOI: 10.1111/j.1756-1051.1993.tb00080.xView Article Google Scholar

Suto, Y., Ohtani, S. 2018 Two new species of foliicolous lichenized Ascomycota, Asterothyrium sasae (Asterothyriaceae) and Gyalectidium shimanense (Gomphillaceae) from Shimane-ken, western Japan. Lichenology 17(2): 35–44.

Tamura, K. 1992 Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Mol. Biol. Evol. 9(4):678–687.
DOI: 10.1093/oxfordjournals.molbev.a040752View Article Google Scholar

Thor, G., L?cking, R., Matsumoto, T. 2000 The foliicolous lichens of Japan. Symb. Bot. Upsal. 32(3): 1–72.van den Boom, P.P.G. 2021 Foliicolous lichens and their lichenicolous fungi in Macaronesia and atlantic Europe. Bibl. Lichenol. 111. 197 pp.

Vilgalys, R., Hester, M. 1990 Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J. Bacteriol. 172(8): 4238–4246.
DOI: 10.1128/jb.172.8.4238-4246.1990View Article Google Scholar

Wang, W.C., Sangvichien, E., Wei, T.Z., Wei, J.C. 2020 A molecular phylogeny of Pilocarpaceae Zahlbr., including a new species of Tapellaria M?ll. Arg. and new records of foliicolous lichenized fungi from Thailand. Lichenologist 52(5): 377–385.
DOI: 10.1017/S0024282920000328View Article Google Scholar

Xavier-Leite, A.B., C?ceres, M.E.S., Aptroot, A., Moncada, B., L?cking, R., Goto, B.T. 2022 Phylogenetic revision of the lichenized family Gomphillaceae (Ascomycota: Graphidales) suggests post-K–Pg boundary diversification and phylogenetic signal in asexual reproductive structures. Mol. Phylogenet. Evol. 168: 107380
DOI: 10.1016/j.ympev.2021.107380View Article Google Scholar

Zoller, S., Scheidegger, C., Sperisen, C. 1999 PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist 31(5): 511–516.
DOI: 10.1006/lich.1999.0220View Article Google Scholar