Research Paper
Multigene genealogy and morphology unveil one new genus, one new species and one new combination of boletoid mushrooms (Boletaceae) from Indian Himalaya
Kanad Das, Sudeshna Datta, Aniket Ghosh, Alfredo Vizzini
Published on: 05 February 2025
Page: 147 - 155
DOI: 10.6165/tai.2025.70.147
Abstract
The present study from the state of Uttarakhand (India) describes the new genus Singeroboletus in the subfamily Suillelloideae (=“Pulveroboletus group”) (Boletaceae, Boletales). This genus is supported by morphological and multigene molecular data. At present this novel genus is represented only by two Asian species, Singeroboletus hainanensis comb. nov. (based on Butyriboletus hainanensis) and S. himalayanus sp. nov. Detailed morphology, illustrations, micromorphological drawings and multigene molecular phylogenetic inferences are presented for S. himalayanus.
Keyword: Agaricomycetes, Fagaceae, molecular phylogeny, Singeroboletus, taxonomy, Uttarakhand
Literature Cited
Arora, D., Frank, J.L. 2014 Clarifying the butter Boletes: a new genus, Butyriboletus, is established to accommodate Boletus sect. Appendiculati, and six new species are described. Mycologia 106(3): 464–480.
DOI: 10.3852/13-052View Article
Google Scholar
Biketova, A.Yu., Gelardi, M., Smith, M.E., Simonini, G., Healy, R.A., Taneyama, Y., Vasquez, G., Kov?cs, ?., Nagy, L.G., Wasser, S.P., Peintner, U., Nevo, E., Bunyard, B.A., Vizzini, A. 2022 Reappraisal of the genus Exsudoporus (Boletaceae) worldwide based on multi-gene phylogeny, morphology and biogeography, and insights on Amoenoboletus. J. Fungus 8(2): 101.
DOI: 10.3390/jof8020101View Article
Google Scholar
Capella-Guti?rrez, S., Silla-Mart?nez, J.M., Gabald?n, T. 2009 trimAl, a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 25(15): 1972–1973.
DOI: 10.1093/bioinformatics/btp348View Article
Google Scholar
Chakraborty, D. 2022 First record of Boletus himalayensis (Basidiomycota, Boletaceae) from Kalatop Wildlife Sanctuary, Himachal Pradesh, India. Kavaka 58(2): 17–21.
Das, K., Datta, S., Singh, U., Ghosh, A., Chakraborty, D. 2023b Multigene molecular phylogeny and morphological evidence reveal one new species and three new records of boletoid fungi from India. Nelumbo 65(2): 1–18
DOI: 10.20324/nelumbo/v65/2023/173225View Article
Google Scholar
Das, K., Ghosh, A., Chakraborty, D., Datta, S., Bera, I., Layola, M.R., Banu, F., Vizzini, A., Wisitrassameewong, K. 2023a Four novel species and two new records of Boletes from India. J. Fungus 9(7): 754.
DOI: 10.3390/jof9070754View Article
Google Scholar
Drummond, A.J., Ashton, B., Buxton, S., Cheung, M., Cooper, A., Heled, J., Kearse, M., Moir, R., Stones-Havas, S., Sturrock, S., Thierer, T., Wilson, A. 2010 Geneious v5.1. Available from http://www.geneious.com
Edler, D., Klein, J., Antonelli, A., Silvestro, D. 2021 raxmlGUI 2.0: a graphical interface and toolkit for phylogenetic analyses using RAxML. Methods Ecol. Evol. 12(2): 373–377.
DOI: 10.1111/2041-210X.13512View Article
Google Scholar
Gardes, M., Bruns, T.D. 1993 ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mole. Ecol. 2(2): 113–118.
DOI: 10.1111/j.1365-294X.1993.tb00005.xView Article
Google Scholar
Hall, T.A. 1999 BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95–98.
Kirk, P.M., Cannon, P.F., Minter, D.W., Stalpers, J.A. 2008 Ainsworth & Bisby’s Dictionary of the Fungi (10th edn). CABI, UK, 771 pp.
DOI: 10.1079/9780851998268.0000View Article
Kornerup, A., Wanscher, J.H. 1978 Methuen handbook of colour, 3rd ed., Eyre Methuen Ltd., London, UK.
Kumar, S., Stecher, G., Tamura, K. 2016 MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33(7): 1870–1874.
DOI: 10.1093/molbev/msw054View Article
Google Scholar
Lanfear, R., Frandsen, P.B., Wright, A.M., Senfeld, T., Calcott, B. 2017 PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Mol. Biol. Evol. 34(3): 772–773.
DOI: 10.1093/molbev/msw260View Article
Google Scholar
Liang, Z-Q., Dong-Yu, A.N., Jiang, S., Su, M-S., Zeng, N.K. 2016 Butyriboletus hainanensis (Boletaceae, Boletales), a new species from tropical China. Phytotaxa 267(4): 256–262.
DOI: 10.11646/phytotaxa.267.4.2View Article
Google Scholar
Liu, Y.J., Whelen, S., Hall, B.D. 1999 Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Mol. Biol. Evol. 16(12): 1799–1808.
DOI: 10.1093/oxfordjournals.molbev.a026092View Article
Google Scholar
Matheny, P.B. 2005 Improving phylogenetic inference of with RPB1 and RPB2 nucleotide sequences (Inocybe; Agaricales). Molecular. Phylogenetics and. Evol. 35(1): 1–20.
DOI: 10.1016/j.ympev.2004.11.014View Article
Google Scholar
Mushtaq, F., Malik, W.S., Chakraborty, D., Bhat, M.H., Vyas, A., Das, K. 2022 Veloporphyrellus latisporus, a new generic record for India. Mycotaxon 137(4): 953–962.
DOI: 10.5248/137.953View Article
Google Scholar
Rambaut, A., Suchard, M.A., Xie, D., Drummond, A.J. 2014 Tracer version 1.6. Available from: http://beast.bio.ed.ac.uk/tracer.
Rehner, S.A., Buckley E. 2005 A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97(1): 84–98.
DOI: 10.3852/mycologia.97.1.84View Article
Google Scholar
Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., H?hna, S., Larget, B., Liu, L., Suchard, M.A., Huelsenbeck, J.P. 2012 MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61(3): 539–542.
DOI: 10.1093/sysbio/sys029View Article
Google Scholar
Tremble, K., Halling, R.E., Henkel, T. W., Moncalvo, J-M., Dentinger, B.T.M. 2023 Nomenclatural novelties. Index Fungorum 555: 1.
Tremble, K., Henkel, T. W., Bradshaw, A., Domnauer, C., Brown, L., Th?m, L.X., Furci, G., Aime, C., Moncalvo, J-M., Dentinger, B.T.M. 2024 A revised phylogeny of Boletaceae using whole genome sequences. Mycologia 116(3): 392–408.
DOI: 10.1080/00275514.2024.2314963View Article
Google Scholar
White, T.J., Bruns, T., Lee S., Taylor, J. 1990 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M. A., D. H. Gelfand, J. J. Sninsky and T. J. White. (eds.), PCR Protocols: a guide to method and applications. Academic Press, San Diego. pp. 315–322.
DOI: 10.1016/B978-0-12-372180-8.50042-1View Article
Google Scholar
Wu, G., Feng, B., Xu, J. P., Zhu, X.T., Li, Y. C., Zeng, N. K., Hosen M.I., Yang, Z.L. 2014 Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae. Fungal Divers. 69(1): 93–115.
DOI: 10.1007/s13225-014-0283-8View Article
Google Scholar
Wu, G., Li, H-J., Horak, E., Wu, K., Li, G-M., Yang, Z. L. 2023a New taxa of Boletaceae from China. Mycosphere 14(1): 745–776.
DOI: 10.5943/mycosphere/14/1/9View Article
Google Scholar
Wu, G., Wu, K., Halling, R.E., Horak, E., Xu, J., Li, G-M., Lee, S., Pecoraro, L., Flores, R. A., Ebika, S.T.N., Aouali, S., Persiani, A.M., Yorou, N.S., Xu, X., Feng, B., Li, Y-C., Yang, Z.L. 2023b The rapid diversification of Boletales is linked to Early Eocene and Mid-Miocene Climatic Optima. bioRxiv preprint. https://doi.org/10.1101/2023.10.24.563795
Zeng, Y-P., Huang, J-Y., Tu, L., Zhao, K. 2024 Complete mitochondrial genome sequence of Butyriboletus hainanensis (Boletales, Basidiomycota). Mitochondrial DNA, Part B. Resour. 9(1): 46–49.
DOI: 10.1080/23802359.2023.2300473View Article
Google Scholar