Abstract

A rapid micropropagation system was developed for Ceropegia bulbosa Roxb. Var. bulbosa. First five nodes of ~ 1.0 cm each, harvested from young healthy shoots from garden raised plants were cultured on B5 medium supplemented with different concentrations of BA and AdS each in combination with 0.05 mg/L NAA. Multiple shoot formation of upto 12 shoots was observed within one week in presence of BAP (3 mg/L) and NAA (0.05 mg/L). Shoots were multiplied by subculture on the same medium. Shoots of 3-4 cm length were rooted in medium supplemented with 2 mg/L IBA. The rooted plantlets were hardened and successfully established in pots at 70% success rate. In vitro flowering was observed at 0.5 mg/L BA+1 mg/L GA3. Shoots transferred to the medium containing kinetin (0.05 mg/L) + IBA (2 mg/L) showed microtubers in 28 days while the shoots cultured in presence of 1 mg/L GA3 +0.5 mg/L of BA showed 76% flowering leading to seed production and 65% of the seeds germinated in successive generations.

中文摘要

本研究提供Ceropegia bulbosa Roxb. Var. bulbosa 一種快速的微體培養系統。取自溫 室栽種植珠之年青健康枝稍的前五節大約各1 公分長的莖節，將之培養於B5 培養基中，其內含不同濃度之BA 與AdS，並均含有0.05 mg/L 的NAA。在含有BAP (3mg/L)與NAA (0.05 mg/L) 的培養基中，一星期內即可觀察到多至12 個再生枝稍，並且在同一培養基的繼代培養中，芽可再增殖。約3-4 cm 長之枝稍可在含有2 mg/L 之IBA 培養基中發根，此發根的小苗可移植於花盆中，成功率約為70%。在BA (0.5 mg/L)與GA3 (1 mg/L)的培養基中可觀察到試管中開花。枝稍移植到含kinetin (0.05 mg/L)與IBA (2 mg/L) 的培養基中，28 天後可觀察到小塊莖，而枝稍培養於GA3 (1 mg/L)與BA (0.5 mg/L) 的培養基中則有76%的枝稍開花，並可形成種子，其種子萌芽率65%。

Keyword: Ceropegia bulbosa Roxb. Var. bulbosa, Medicinal plant, Micropropagation, In vitro flowering