Abstract

Phloem exudates leaking from Luffa cylindrica fruits by a simple razor blade cutting were mixed with the extraction medium containing antioxidant and rapidly separated by 2-dimentional gel, i. E., native and sodium dodecylsulfate(SDS) polyacrylamide gel electrophoresis(PAGE). A major protein of 85kDa occupying 28.2% of total protein had 5 bands on denatured gel. One of them with molecular weight at 42.5kDa was complementally identified by reversed-phase high performance liauid chromatography and silver stained-band on SDS-gel.

中文摘要

絲瓜果實經刀片輕切裂口，塗上含抗氧化劑的萃取液後，其與韌皮部溢流物一起收集。以混合液經活性與變性(SDS)的兩次電泳分析。一維分離獲得85kDa的變性蛋白質在高效能電泳色層分析顯示單一峰帶，而在變性銀染色膠上僅呈一蛋白質帶。

Keyword: Yung-Reui Chen